Overview
This page describes the data levels, metadata attributes, and file structure for bulk RNA sequencing.
Description of Assay
Bulk RNA sequencing identifies the average gene expression profile of a biological sample.
Metadata Levels
The defined metadata leverages existing common data elements from the Genomic Data Commons (GDC). The HTAN data model currently supports Level 1, 2 and 3 RNA sequencing data:
|
Level Number |
Definition |
Example Data |
|
1 |
Unaligned reads |
FASTQ |
|
2 |
Aligned reads |
BAM |
|
3 |
Gene level expression, unnormalized |
Gene & isoform expression-level data (.csv) |