This page describes the data levels, metadata attributes, and file structure for bulk RNA sequencing.
Bulk RNA sequencing identifies the average gene expression profile of a biological sample.
The defined metadata leverages existing common data elements from the Genomic Data Commons (GDC). The HTAN data model currently supports Level 1, 2 and 3 RNA sequencing data:
| Level Number | Definition | Example Data |
|---|---|---|
| 1 | Unaligned reads | FASTQ |
| 2 | Aligned reads | BAM |
| 3 | Gene level expression, unnormalized | Gene & isoform expression-level data (.csv) |